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Role of AREB6/ZEB Transcription Factor in Invasive Breast Cancer

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Annual summary rept. 19 Apr 2002-18 Apr 2003

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The major goal of the proposed research is to test the hypothesis that the estrogen-inducible transcription factor AREB6ZEB contributes to the progression of breast carcinoma. In specific, I am investigating whether the expression of AREB6ZEB becomes deregulated from estrogen control and, if so, at what stage of carcinogenesis. To test this, staged breast cancer samples are being collected and assayed by real-time PCR for the level of AREB6ZEB mRNA. Furthermore, I am assessing relative estrogen levels by determining the amounts of pS2 and progesterone receptor mRNA in the biopsies, also by real-time PCR. The use of real-time PCR and the measurement of pS2 and progesterone receptor mRNAs represent changes in strategy from the original proposal. These changes were made because we were informed that serum estrogen levels were an unreliable measure of tissue estrogen levels especially in postmenopausal women, because we were having difficulty collecting paired biopsy samples and serum, and because a real-time PCR machine became available. In addition, we are also measuring the amount of another family member ZEB-2, which has been implicated recently in invasion. At this point, I have virtually all the samples and reagents in hand to complete the proposed studies in the upcoming year.

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  • Genetic Engineering and Molecular Biology
  • Medicine and Medical Research

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