Prostasin Serine Protease as a Breast Cancer Invasion Marker and a Metastasis Suppressor
Annual rept. 8 Apr 2002-7 Apr 2003
UNIVERSITY OF CENTRAL FLORIDA ORLANDO
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We have previously shown that membrane-anchored prostasin serine protease was an in vitro suppressor of tumor cell invasion 1-3 . The expression of prostasin is down-regulated in prostate cancers 2, and invasive cancer cell lines of the human prostate and breast 1, 3. We hypothesized that the down-regulation of prostasin is causal to breast cancer invasion and metastasis in vivo. Two highly invasive human breast cancer cell lines, the MDA-MB-231 and MDA-MB-435, were transfected with a prostasin cDNA plasmid to restore prostasin expression. The transfectants of MDA-MB-231 were used in the nude mice model to assess the metastatic potential. The results demonstrated a statistically significant difference of metastasis between cells that express prostasin and the control cells. Prostasin expression in the transfected cells was analyzed by immunocytochemistry and clones that express prostasin more uniformly were obtained for repeat experiments in nude mice for the purpose of maximizing the effect of prostasin.
- Anatomy and Physiology
- Medicine and Medical Research