The Role of Sigma Receptor in Breast Cancer
Annual summary 1 Apr 2002-31 Mar 2003
M D ANDERSON CANCER CENTER HOUSTON TX
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Task 1 of our proposal is to determine the affect of Sigma 1 receptor activity on breast cancer cell growth and tumorigenesis in vitro and in vivo. This work includes assessment of expression of Sigma 1 receptor in established breast cancer cell lines and normal breast epithetial cultures by RT-PCR. We also plan to study the effect of Sigma receptor activation or inhibition on cell proliferation, and apoptosis in vitro by MTT assay, colony formation assay and flow cytometry on different cell lines. To further evaluate the role of Sig 1 R we will generate stable transfectants of Sigma receptor and determine the biological effects of forced Sigma receptor expression. Task 2 of our proposal is to determine the expression pattern of Sigmal 1 receptor in human breast cancer. This work includes generation of a monoclonal antibody against Sigma receptor assessment of its staining characteristics in human tissues and immunohistochemistry staining of paraffin embedded sections of breast tumors and normal breast samples.
- Anatomy and Physiology
- Medicine and Medical Research