Accession Number:

ADA416629

Title:

Ex Vivo Expansion of HER2-Specific T Cells for the Treatment of HER2-Overexpressing Breast Cancer

Descriptive Note:

Annual summary rept. 3 Apr 2000-2 Apr 2003

Corporate Author:

WASHINGTON UNIV SEATTLE

Personal Author(s):

Report Date:

2003-05-01

Pagination or Media Count:

154.0

Abstract:

Adoptive T cell therapy has the potential to eradicate existing malignancy in humans. I have been investigating the efficacy of adoptive T cell therapy at eradicating malignancy in the neu-transgenic mouse model of human breast cancer. Helper peptides of neu have been identified to which T helper cell lines can be generated. Cell lines derived using these peptides, were tested for the ability to eradicate existing bulky malignancy. T cell injection resulted in a partial tumor response. I have been investigating how to improve efficacy. Polyclonal T cell lines recognizing multiple epitopes are more effective. I have observed that immunosuppressive T cells associate with these breast tumors. Prior elimination of these cells nay improve outcome. A monoclonal antibody therapy strategy has also been developed that will be tested in combination with adoptive T cell transfer. This is an adaptation of this murine model to better reflect current strategies in humans i.e. Herceptin Techniques for optimal ex vivo expansion of human HER-2neu-specific T cells are also continuing to be developed. Antigen-specificity of cultures can be preserved following rapid expansion with anti-CD3CD28 beads. The findings in the animal model and ex vivo expansion of human T cells will be directly translated to human clinical trials of adoptive T cell therapy.

Subject Categories:

  • Anatomy and Physiology
  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE