Gene Methylation in Prostate Cancer
Annual summary 1 Dec 2001-30 Nov 2002
AMERICAN UNIV WASHINGTON DC
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Background NKX3. 1 is a homeoprotein with prostate-specific expression in adults. Loss of NKX3.1 correlates with prostate cancer progression. Loss of heterozygosity affects NKK3.1 in about 80 of prostate cancers. This project focuses on DNA methylation of the NKK3.1 gene promoter in prostate cancer cell lines and tissues. Materials and methods DNA was analyzed by methylation-specific PCR and sequencing of bisulfite-treated DNA. We also treated cultured cells with the methylation ihhibitor 5-azacytidine and the histone deacetylase inhibitor, trichostatin A. Results There was no effect of 5-azacytidine or trichostatin A on NKY3.1 expressionin cultured cells. By bisulfite modified DNA sequencing, we identified some methylated or partial methylated CpU islands in -1056 to 1172 of NKX3.1 gene. Some of tbem are selected to study their methylated situation in human prostate cancer tissues. We also study the effect of Sp transcription factor family on the expression of NKX.3.l, our results showed Spl and 5p3 did not influence on the expression of NKX3. 1. Conclusion Some CpG islands in NKX3.l gene are methylated or partial methylated.
- Genetic Engineering and Molecular Biology
- Anatomy and Physiology
- Medicine and Medical Research