Signaling Pathways Controlling the Growth and Proliferation of Drosophilae Perineurial Glial Cells
Annual rept. 1 May 2002-30 Apr 2003
RICE UNIV HOUSTON TX
Pagination or Media Count:
The long term goals of this research are to understand the mechanisms by which NF1 and its partners control growth using the Drosophila peripheral nerve. This system is advantageous because we can apply a number of powerful molecular genetic methodologies that are not available in other systems. The aims of this project address three specific aspects of growth control. First, we asked if NF1 acts downstream of a G protein to exert its effects. Based on initial experiments from this grant and related experiments from a different grant, we obtained evidence that NF1 has dual, opposing roles in glial growth control. Thus, our original hypothesis is incomplete and must be remodeled. Second, we tested further the hypothesis that motor neuron activity affects perineurial glial growth. So far, our experiments on triple mutants exhibiting extremely elevated neurotransmitter signaling failed to find significant effects on glial growth. Further experiments are in progress. Third, we asked if perineurial glial growth can be genetically uncoupled from perineurial glial proliferation. We found that expression of a constitutively active Ras does, in fact, cell nonautonomously increase perineurial glial nuclei number, but this increase is not required for the growth effects observed in chromosomal mutants.
- Anatomy and Physiology
- Medicine and Medical Research