Accession Number:

ADA416133

Title:

Establish an in Vitro Model for the Study of NF2 Gene Function

Descriptive Note:

Final rept. 1 Oct 1999-30 Sep 2002

Corporate Author:

HOUSE EAR INST LOS ANGELES CA

Personal Author(s):

Report Date:

2002-10-01

Pagination or Media Count:

8.0

Abstract:

Mutation analyses of the NF2 gene from NF2 patients suggest that the inactivation of the NF2 gene, and the consequent lack of gene product protein known as MerlinSchwannomin is the primary cause of this disease. Within the three years period, we plan to develop a laboratory protocol to establish Schwann cell culture using surgical specimens from NF2 patients, and compare the tumor cells from patients with different NF2 gene mutations. In addition, we want to extend the life span of primary culture cells by immortalizing them using a retrovirus which we engineered. This will not only ensure the reproducibility of results within the NF2 community, but also provide scientists greater access to certain materials for the study of biologic function of Schwann cell and also important for testing therapeutic approaches. During the pass 12 months period, PI., Dr. Gene Hung has relocated himself from House Ear Institute to Arena Pharmaceuticals on March, 2002. An application for grant sponsorship transfer and additional request for a no cost extension 12 months to this grant have been submitted.

Subject Categories:

  • Genetic Engineering and Molecular Biology
  • Anatomy and Physiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE