Improving Blood Monitoring of Enzymes as Biomarkers of Risk From Anticholinergic Pesticides and Chemical Warfare Agents
Annual rept. 24 Sep 2001-23 Sep 2002
CALIFORNIA UNIV DAVIS
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Blood biomarkers are an important way to monitor exposure to anticholinergic pesticides and chemical warfare CW agents and to establish whether some are at greater risk than others from exposure to them. Many clinical and research laboratories use the colorimetric Ellman assay based on the hydrolysis of acetylthiocholine. CHPPM US Army Center For Health Promotion and Preventive Medicine uses a slower delta pH method based on that of Michel to monitor more than 25,000 DOD personnel each year. This year blood drawn under the appropriate regulations by CHPPM was centrifuged and hydrolyzed before being assayed by the delta pH method with acetylcholine as substrate. Paired samples were sent to UC Davis to be assayed with acetylthiocholine by the Ellman method. UC Davis samples were diluted with buffer and run with and without quinidine to separate activities due to acetylcholinesterase AChE and non-specific cholinesterase BuChE. Slopes of pH vs Ellman results for three of five sets of samples had similar slopes and yielded correlations of r2 of 0.74 to 0.8. Work continues on comparisons to establish critical assay conditions and Ellman equivalents of the delta pH assay.
- Medicine and Medical Research
- Chemical, Biological and Radiological Warfare