The Role of Bone Marrow-Derived Stem Cells on Mammary Tumor Angiogenesis, Growth and Metastasis, and the Influence of VEGF-A and PIGF on their Recruitment
Annual rept. 15 Apr 2002-14 Apr 2003
BETH ISRAEL DEACONESS MEDICAL CENTER BOSTON MA
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This proposal is designed to further our understanding of the role of bone marrow derived endothelial precursor cells to mammary-tumor angiogenesis, tumor growth and progression. We have designed a unique animal model system to carefully study the involvement of these cells in mammary tumor progression and tumor angiogenesis. The potential importance of these cells to cancer warrants the analysis of these bone marrow derived vascular cells to as many types of cancer settings as possible. Moreover, studies have shown that these endothelial precursor cells are regulated by estrogen in endometrial angiogenesis, suggesting they may have an important role in the vascularization of mammary tumors and may be affected by anti-estrogen therapy. Specific Aims 1 To determine the relative contribution of bone marrow derived endothelial cells to naturally occurring mammary tumors during stages of early, late and metastatic tumor growth. 2 To determine the impact of the angiogenic cytokines VEGF-A and PIGF on tumor recruitment of bone marrow derived endothelial cells. 3 To determine if estrogen impacts on the recruitment of bone marrow derived endothelial cells to mammary tumors.
- Anatomy and Physiology
- Medicine and Medical Research