Accession Number:

ADA415609

Title:

IGF-Regulated Genes in Prostate Cancer

Descriptive Note:

Annual rept. 1 Feb 2002-31 Jan 2003

Corporate Author:

OREGON HEALTH SCIENCES UNIV PORTLAND

Personal Author(s):

Report Date:

2003-02-01

Pagination or Media Count:

6.0

Abstract:

We hypothesized that genes that are differentially expressed as a result of the decreased IGF-I receptor gene expression seen in metastatic prostate cancer contribute to prostate cancer progression. IGF-I receptor target genes may include metastasis-promoting or suppressing genes that could constitute valuable diagnostic markers or therapeutic targets. To evaluate this hypothesis, we proposed three specific aims I Identification of genes that are differentially regulated in otherwise isogenic metastatic vs. non-metastatic prostate epithelial cells 2 Identification of proteins that are differentially secreted in the cell lines used in aim 1, and 3 Assessment of the differential expression of these genes and gene products in laser-microdissected samples. We have used microarray gene profiling to characterize differentially expressed genes and have used SELDI-TOF mass spectrometry to identify proteins that are differentially secreted into conditioned media. We have additionally initiated a 3-dimensional culture system to grow prostate cells in a microgravity environment that more accurately replicates in vivo cell organization and phenotype. We are now preparing to quickly repeat our microarray and SELDI-TOF analyses in 3-D culture before proceeding to the validation of differential expression in clinical samples using prostate tissue arrays.

Subject Categories:

  • Anatomy and Physiology
  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE