Accession Number:

ADA415604

Title:

Structural and Functional Analysis of Androgen Receptor-DNA Interactions

Descriptive Note:

Annual rept. 1 Feb 2002-31 Jan 2003

Corporate Author:

DUKE UNIV MEDICAL CENTER DURHAM NC

Personal Author(s):

Report Date:

2003-02-01

Pagination or Media Count:

17.0

Abstract:

Our objective is to use X-ray crystallography to determine the basis for specific interactions between the Androgen receptor and its DNA targets, in order to understand how the receptor can recognize two different bipartite DNA response elements with diametrically opposing arrangements. Our research has now identified a variant of the AR DNA binding domain that yields large, single lattice crystals when bound to a direct repeat response element. These crystals diffract to beyond 3.5 A using home source X-rays and are suitable candidates for solving the structure of the AR dbd-DNA complex. We have thus overcome the significant obstacle in the crystal structure determination. Analysis of the protein-protein and protein-DNA contacts that lead to specific binding could be used to design novel classes of anti-androgens.

Subject Categories:

  • Medicine and Medical Research
  • Radiobiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE