Accession Number:

ADA415600

Title:

Retinol Esterification in Human Breast Cancer Cells

Descriptive Note:

Annual summary rept. 1 Jan 2000-31 Dec 2002

Corporate Author:

CORNELL UNIV MEDICAL COLL NEW YORK

Personal Author(s):

Report Date:

2003-01-01

Pagination or Media Count:

16.0

Abstract:

This study was designed to identify the target genes of retinoids in the normal human mammary epithelial cells HMEC and breast cancer cells. In our early studies, we found that the IL-1Beta gene was a responsive gene to retinoic acid RA and its expression was up-regulated as early as two hours after RA treatment. In the recent studies, we examined the gene expression profiles in the human mammary epithelial cells following RA and 4-oxo-retinol 4-oxo-ROL treatment. Our results indicated that both 4-oxo-ROL 1 micrometer and RA 1 micrometer strongly inhibited the proliferation of HMEC. The microarray analyses show that a large number of genes, including transcription factors, nuclear receptor co-regulators, cell cycle regulatory proteins and protein kinases, are regulated by RA and 4-oxo-ROL. Although many genes were found to be regulated by both RA and 4-oxo-ROL, 4-oxo-ROL was not converted to RA in HMEC, which suggests that 4-oxo-ROL regulates gene expression by a mechanism different from RA.

Subject Categories:

  • Anatomy and Physiology
  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE