Accession Number:

ADA415301

Title:

Nanoparticle-Mediated p53 Gene Therapy for Breast Cancer

Descriptive Note:

Annual summary 15 Mar 2002-14 Mar 2003

Corporate Author:

NEBRASKA UNIV AT OMAHA COLL OF MEDICINE

Report Date:

2003-04-01

Pagination or Media Count:

39.0

Abstract:

The effect of different formulation parameters on nanoparticle-mediated gene transfection in vitro was studied Nanoparticles encapsulating plasmid DNA encoding fire fly luciferase were formulated using poly lactide PLA and poly dI-lactide co-glycolide PLGA polymers of different composition and molecular weight A multiple emulsion solvent evaporation method with polyvinyl alcohol PVA as an emulsifier was used to formulate DNA-loaded nanoparticles Transfection of various nanoparticles formulations was determined in breast cancer MCF-7 cell line. Nanoparticles formulated with PLA demonstrated relatively lower transfection than those formulated with PIGA because of higher DNA release from PLGA nanoparticles than that from PLA nanoparticles.

Subject Categories:

  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE