Accession Number:

ADA415172

Title:

DNA Replication During Conjugal Transfer of R1162

Descriptive Note:

Final rept. 16 Apr 2001-15 Apr 2003

Corporate Author:

TEXAS UNIV AT AUSTIN

Personal Author(s):

Report Date:

2002-01-01

Pagination or Media Count:

21.0

Abstract:

Conjugative DNA transfer is an important mechanism for the acquisition of multiple drug resistance by bacteria. The role of DNA synthesis in this process was examined under conditions where vegetative replication was strictly prevented. There was more than one round of transfer from a donor cell containing a single plasmid molecule, indicating replacement strand synthesis by a conjugation-specific mechanism. A significant time lag between rounds of transfer was detected, arguing against the spooling of DNA into recipients by a rolling-circle replication.

Subject Categories:

  • Biochemistry

Distribution Statement:

APPROVED FOR PUBLIC RELEASE