Accession Number:

ADA413966

Title:

An Epidermal Biosensor for Carcinoembryonic Antigen

Descriptive Note:

Final rept. 1 Jul 1999-31 Dec 002

Corporate Author:

VETERANS ADMINISTRATION MEDICAL CENTER WEST HAVEN CT

Personal Author(s):

Report Date:

2003-01-01

Pagination or Media Count:

21.0

Abstract:

The goal of this grant was to develop a prototype epidermal biosensor for carcinoembryonic antigen CEA An epidermal biosensor was conceived as a new approach for the early continuous, in vivo detection of the onset of disease by the using genetically modified skin cells to respond to molecules secreted by tumor cells. The research we have conducted has allowed us to conclude that human keratinocytes in vitro can be engineered to express a chimeric cell surface receptor and that these modified cells could recognize and bind CEA Technical problems in generating chimeric receptor constructs were resolved but we were unable to create a chimeric cell surface receptors that had domains that would bind and respond to CEA in human keratinocytes In the absence of supplemental finding, we were unable to pursue other avenues for constructing cell surface receptors that would introduce into keratinocytes the capacity to act as in vivo biosensors. We remain confident that such chimeric receptors can be generated to respond to tumor antigens such as CEA. We believe that keratinocyte biosensors could be effective early detection systems and play an important role in the management of breast cancer

Subject Categories:

  • Anatomy and Physiology
  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE