Oxidized DNA Bases in Breast Cancer
Annual summary 1 Aug 1999-31 Jul 2002
NEW YORK UNIV NY SCHOOL OF MEDICINE
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Recently more studies have correlated the elevated 5-hydroxymethyl-2-deoxyuridine HMdU - an oxidized DNA base levels with an increased risk of cancer when compared to controls. HMdU has been proposed to be a biomarker for breast cancer risk Although methods are available, but requires larger sample sizes between 50- 100 micrograms DNA, which limits its application for HMdU quantitation in relatively smaller sample size We have developed a sensitive method to detect and quantitate HMdU using fluorescent coumarin derivative to label HMdU and other nucleosides.
- Medicine and Medical Research