Identification of a Gene on Chromosome 18q21 Involved in Suppressing Metastatic Prostate Cancer
Annual rept. 1 Dec 2001-30 Nov 2002
TEXAS UNIV HEALTH SCIENCE CENTER AT SANANTONIO
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Previous studies using allelic imbalance Al have implicated numerous chromosomal regions as potential locations for tumor suppressor genes involved in prostate cancer. In primary prostate cancer the most frequent losses occur at 8p, 1Oq, 11p, 16q, 17p, 18q, and 21. In addition, in metastatic prostate cancer there is an increased frequency of Al at 18q, compared to primary cancer, and there are two distinct regions of loss 7 and 6 centimorgans in size, respectively on 18q associated with the metastatic samples. In order to identify. a smaller region of 18q that harbors a gene involved in metastatic prostate cancer, we have constructed contigs of bacterial artificial chromosomes BACs that encompass the two regions of loss. DNA from these BAC clones has been spotted onto slides by Spectral Genomics. These microarray slides are in the process of being hybridized with DNA from prostate tumors at various stages of cancer progression. These microarray experiments will provide more information about chromosome 18 losses than the previous Al experiments, since there is no limitation for a particular sample to be heterozygous for an 18q marker. These microarray data will help to pinpoint the location of the proposed metastasis suppressor gene.
- Genetic Engineering and Molecular Biology
- Medicine and Medical Research