Tropomyosin-1: A Putative Tumor Suppressor and a Biomarker of Human Breast Cancer
Annual summary rept. 1 Oct 2001-30 Sep 2002
WAKE FOREST UNIV WINSTON-SALEM NC SCHOOL OF MEDICINE
Pagination or Media Count:
Previous research from this laboratory indicated that 1 the expression of Tropomyosin-1 TM1 a microfilament-associated protein, is abolished from many human breast carcinoma cells, and 2 that TM1 is suppressor of the malignant transformation. These data led to the hypothesis that TM1 plays an important role in mammary carcinogenesis. Therefore, we investigated whether TM1 could serve as a biomarker of breast cancer, and TM1 could function as a suppressor of the malignant growth of breast cancer cells. In situ hybridization experiments showed that TM1 expression is downregulated in breast tumors Objective 1. We demonstrated that TM1 is a suppressor of the malignant growth phenotype of MDA MB 231 cells, indicating that TM1 is a general suppressor of the transformed growth Objective 2. To assess the structure-function relationship of tumor suppression by TM1, chimeras of TM1 and TM2 which is not a tumor suppressor are constructed. In contrast to TM1, TM2 is not a suppressor neoplastic growth of breast cancer cells. The chimeras of TM1 and TM2 will be transfected into MDA MB 231 cells Objective 4 .
- Anatomy and Physiology
- Medicine and Medical Research