Control of Metastasis-Associated Gene Expression by Cell-Surface Beta-1, 6 Branched Oligosaccharide Expression
Final rept. 1 Jun 2001-31 May 2002
NEBRASKA UNIV MEDICAL CENTER OMAHA
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Metastatic breast cancer cells express cell-surface Beta-1,6 branched oligosaccharide structures, which are reducedabsent in normal breast tissue. The biosynthesis at these structures is initiated by the Golgi-localized glycosyltransferase N-Acetylglucosamlnyl- transferase V GlcNAc-T V. The altered expression or GlcNAc-T V in animal mammary cancer models also influences metastasis. Furthermore, altered transcription of metastasis- associated genes has also been observed in cells with a reduced expression at cell-surface Beta-l,6 branched oligosaccharide expression. The aim at this project is to characterize the altered expression of mRNA in mouse and human mammary cancer-derived cell lines as a function at altered Beta-l,6 branched oligosaccharide expression. This will be accomplished by using DNA Microarray technology to assess the mRNA levels in cell lines transfected with GlcNAc-T V expression vectors. After identification of mRNA molecules that are altered by changes elevation or reduction in cell-surface Beta-l,6 branched oligosaccharide expression, the kinetics at induction will be characterized using cell lines with GlcNAc-T V under the control at an inducible promoter.
- Genetic Engineering and Molecular Biology
- Anatomy and Physiology
- Medicine and Medical Research