NF1 - Regulated Adenylyl Cyclase/cAMP Pathways
Final rept. 1 Sep 1999-1 Sep 2002
COLD SPRING HARBOR LAB NY
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This project takes Drosophila NF1 mutants and mouse Nf1 mutants as models to investigate NF1-dependent regulation of the cAMP pathway. The study is intended to expand the knowledge of the genes that contribute to NF beyond the GAP-related domain in NF 1 In the last year, our work has been mainly focused on two aspects. First, the effort has been devoted to establish biochemically how G-protein-dependent activation of adenylyl cyclase AC activity is regulated by NF1 in Drosophila. Second, whether a similar biochemical regulation of AC activity can be identified in vertebrates These studies have led to a finding that G-protein-dependent activation of AC consists of two components one is classically described NF 1-independent one while the other is NF 1-dependent Accumulated evidence from our last years effort support that a similar NF-dependent mechanism can be observed in vertebrates We are continuing to pursue a molecular understanding of this regulation and whether this pathway contributes to pathogenesis of NF1.
- Medicine and Medical Research