USF-1 as an Inhibitor of Mammary Gland Carcinogenesis
Annual rept. 1 Sep 2001-31 Aug 2002
BAYLOR COLL OF MEDICINE HOUSTON TX
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The hypothesis tested in these studies is that overexpression of USF in the mammary glands of transgenic mice will inhibit myc-dependent tumorigenesis. Expression of a FLAG-tagged form or USF-2 was targeted to the mammary gland under the control of the mouse mammary tumor virus mmtv long terminal repeat. Of eight lines of transgenic mice that were generated, one demonstrated expression of flag-tagged USF-2 in the lactating mammary gland at levels 12-fold over that of endogenous USF-2. Evaluation of tumorigenesis in these mice n17 out to 453 days of age suggests that USF-2 when overexpressed by itself is not oncogenic. In contrast, 72 of mmtv-myc mice n18 analyzed have developed mammary tumors with an average latency of 156-33 days.
- Anatomy and Physiology
- Medicine and Medical Research