Accession Number:

ADA411739

Title:

Can Labeled Human CD 34+ Lymphocytes be Used to Identify Sites of Angiogenesis Within Growing Tumors

Descriptive Note:

Final rept. 30 Sep 2000-30 Sep 2002

Corporate Author:

STATE UNIV OF NEW YORK AT BUFFALO AMHERST RESEARCH FOUNDATION

Personal Author(s):

Report Date:

2002-10-01

Pagination or Media Count:

9.0

Abstract:

We have proposed that CD34 vascular endothelial progenitor VEP cells in blood can be used to transport therapeutic genes into areas of angiogenesis within growing breast cancer tumors. Our system is tumors MDA-MB 231 grown in SCID mice. It was difficult to grow these tumors. We learned that NK cells and NKT cells had to be eliminated before tumors could grow. CD34 cells were isolated from leukopacks and labeled with a lipophilic fluorescent dye, DiI. Leukopacks do not supply us with enough CD34 cells to further isolate subsets of cells that have phenotypic markers of VEP.

Subject Categories:

  • Biochemistry
  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE