Accession Number:

ADA411664

Title:

Biological Agent Sample Preparation for the Detection and Identification of Multiple Agents by Nucleic Acid-Based Analysis

Descriptive Note:

Final rept. 9 Nov 2001-8 Nov 2002, Phase 2

Corporate Author:

XOHOX INC MENLO PARK CA

Personal Author(s):

Report Date:

2002-12-01

Pagination or Media Count:

22.0

Abstract:

We have designed, developed and tested an instrument prototype for automating the front end of nucleic acid test procedures. The AutoLyser instrument which we have developed, provides fully automated purification of viral, bacterial and human genomic DNA and RNA from clinical samples, cell culture and swabs in as little as 10 minutes, and is capable of purifying between 1 and 8 samples at a time. No manual steps are required during a purification run. Samples are rendered non-infectious and are processed inside a disposable plastic device which can be quickly attached or removed from the instrument by relatively unskilled technical personnel. The possibility of contact with an infectious sample is almost eliminated. Centrifugation is not required since reagents are moved within the sample cartridge by means of precision syringe pumps and reagent selection valves under software control. Purified nucleic acid template is eluted in water and is ready for use in PCR or other downstream operations.

Subject Categories:

  • Biochemistry
  • Chemical, Biological and Radiological Warfare

Distribution Statement:

APPROVED FOR PUBLIC RELEASE