Y Chromosome Aberrations in Prostate Cancer
Annual rept. Phase II. 1 Sep 2001-31 Aug 2002
TEXAS UNIV HEALTH SCIENCE CENTER AT SANANTONIO
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We hypothesize that chromosomal loss of the Y chromosome in prostate cancer is a specific tumorigenic event. Transfer of the Y chromosome by microcell mediated chromosome transfer into PC-3 cells resulted in suppression of tumor growth. However, these PC-3 hybrids containing the Y chromosome did grow in soft agar indicating a lack of correlation between in vitro and in vivo tumor growth. We have isolated clones with fragments of the Y chromosome and found suppression of tumorigenesis correlates with either the short arm of the Y or two small regions on the q arm. The data from the functional assay will be correlated with data from deletions in patient material. We have isolated a complete genomic microarray specific for then Y chromosome. These BAC bacterial artificial chromosomes will be used to perform array CGH Comparative genomic hybridization of prostate tumor samples. Successful identification of a gene on the Y chromosome will provide a marker that may aid in the diagnosis and prediction of prognosis of prostate cancer.
- Anatomy and Physiology
- Medicine and Medical Research