Genetic and Molecular Characterization of Drosophila Brakeless: A Novel Modifier of Merlin Phenotypes
Annual rept. 1 Jul 2001-30 Jun 2002
NORTH CAROLINA UNIV AT GREENSBORO
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In the first year of our grant we have concentrated on determining the nature of scribbler. As presented in our Statement of Work the first specific aim deals with the Molecular and genetic analysis of bks sbb function. We proposed four experimental approaches to this problem and have started on three. In a screen of 5000 mutant second chromosomes, we have identified five new alleles of scribbler. The new alleles of scribbler are all lethal in trans with the null scribbler mutation, sbb4 however, there is a range of genetic interaction phenotypes between these alleles and mutations in Merlin. We are currently determining the molecular lesions in these scribbler mutations and correlating them with their interaction with Merlin. We have also generated eight FLAG tagged mutant versions of scribbler. The transgenics animals are currently being generating. One interesting and intriguing result is that over expression of the larger but not the smaller wild- type isoform of sbb in the Drosophila wing results in overgrowth phenotypes similar to that observed with mutations in Merlin. As part of Specific Aim II, we have also shown a genetic interaction of mutations in Cyclin E with scribbler and Merlin mutations.
- Genetic Engineering and Molecular Biology
- Medicine and Medical Research