Mammary Stromal Effects on Epithelial Differentiation and Expression of ESX and ErbB2 (HER2/neu)
Annual summary rept. 20 Aug 2001-19 Aug 2002
CALIFORNIA UNIV SAN FRANCISCO
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A novel system for studying growth of normal human mammary epithelium in vivo as grafts in athymic nude mice has been developed. The key feature of this model is the reconstitution of the epithelial-stromal interactions required for normal growth and differentiation of the human mammary epithelium, which produces ducts that are comparable to the normal human mammary gland. Human breast epithelial organoids were combined with mammary fibroblasts from mouse or human origin in collagen gels, which were subsequently transplanted under the renal capsule of female nude mice hosts. The resulting grafts showed an increase in the ductal density than observed previously. These ducts expressed appropriate markers for luminal and myoepithelial cells and steroid receptors.
- Anatomy and Physiology
- Medicine and Medical Research