QuEST: A New Approach to Molecular Staging of Tumors
Annual rept. 30 Jul 2001-29 Jul 2002
WASHINGTON UNIV SEATTLE
Pagination or Media Count:
Towards the goal of molecular diagnosis and staging of NF1-related tumors, we proposed to develop and validate a novel PCR-based method, termed QUEST, for rapid and quantitative identification of loci with increases or decreases in genomic copy number in MPNST. We have performed a comprehensive literature search and compilation of the genetic changes in NFl-associated peripheral nerve sheath tumors see manuscript in Appendix. The implications of this analysis are that consistent regions of the genome frequently undergo gains and losses in NF 1-associated MPNST. This phenomenon underscores the validity of our approach and increases the probability that it may be of clinical andor research use. Development and validation of multiple quantitative PCR assays at test and control loci and the SKY karyotypic analysis of tumor cell lines are in progress. These will provide the resources necessary to determine the usefulness of our idea of using QUEST for staging of tumors.
- Medicine and Medical Research