DNA Vaccination of Overcome Peripheral Tolerance
Annual rept. 1 Aug 2002-31 Jul 2002
WAYNE STATE UNIV DETROIT MI
Pagination or Media Count:
The vaccination efficacy of DNA encoding autologous rat ErbB-2 neu or heterologous human ErbB-2 Her-2 is compared in rat neu transgenic mice BALB neuT. Cross reactivity between Her-2 and rat neu was tested initially by immunizing normal mice, twice, i.m. with pEFBosGM-CSF and pCMVE2TM or pCDneuTM. E2TM and neuTM encodes the extracellular ECD and transmembrane TM domains of Her-2 and neu, respectively. Immunized mice were challenged with mammary tumor D2F2 expressing Her-2 D2F2E2 or neu D2F2neu. All mice immunized with E2TM or neuTM rejected tumors expressing the corresponding antigen.
- Medicine and Medical Research