The Functional Role(s) of Neurofibromin During Neural Crest Cell Development
Final rept. 1 aug 2000-31 Jul 2002
CHILDREN'S HOSPITAL OF PHILADELPHIA PA
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This grant proposal set out to determine if neurofibromin plays an important role during early neural crest cell development The objectives of this study were A set-up and maintain a viable colony of Nf - mice to generate Nf , Nf - and Nf -- embryos. B Set up a breeding protocol that provides accurately timed pregnant E9 embryos. C Devise assays to reproducibly assay neural crest cell proliferation and cell death. D Fix, freeze and collect enough E9 Nf , Nf - and Nf -- embryos with which to generate the relevant data. E Serially section the embryos in D and perform the proliferation and cell death assays in C to determine whether or not neurofibromin modulates neural crest cell proliferation and cell death in these embryos. To date objectives A-D have been accomplished. Objective E is ongoing and should be completed over the next 6-9 months.
- Medicine and Medical Research