Accession Number:

ADA411417

Title:

A Novel Monoallelically Expressed Candidate Tumor Suppressor Gene for Breast Carcinogenesis on Chromosome 11p15.5

Descriptive Note:

Annual summary rept. 1 Jul 1999-30 Jun 2002

Corporate Author:

MASSACHUSETTS INST OF TECH CAMBRIDGE

Report Date:

2002-07-01

Pagination or Media Count:

11.0

Abstract:

I have identified a novel Xist-like nuclear transcript 11p-xlnt which is monoallelically expressed and accumulates in the interphase nucleus at its site of transcription. 11p-xlnt is transcribed from within a narrowly defined 500 kb portion of human chromosome 11p15.5 that exhibits loss of heterozygosity LOH in 40 of breast cancers. I have shown that 11p-xlnt is transcribed from within an intron of and antisense to the KvLQT gene and spans at least 40 kb of genomic sequence. Construction and screening of a cDNA library failed to reveal evidence of splicing for this large transcript. While transcription appears to be exclusively from the paternal allele in normal tissues, I have shown that expression occurs from both maternal and paternal alleles in a rhabdomyosarcoma cell line, although expression is found to be monoallelic on a single cell basis by RNA FISH. 11p-xlnt may play a part in local and regional gene regulation at the imprinted domain on chromosome 11p.

Subject Categories:

  • Biochemistry
  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE