Alpha (1, 3) Galactosyltransferase Gene Therapy for Breast Cancer
Annual rept. 1 Jul 2001-30 Jun 2002
HUMAN GENE THERAPY RESEARCH INST DES MOINES IA
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ALPHA1,3GALACTOSYL EPITOPES ALPHA Ga1 expressed on membrane glycoproteins and glycolipids are the major target of the human hyperacute rejection response observed when organs are transplanted from nonprimate donor species. Expression of the alpha1,3galactosyltransferase gene ALPHA1,3GT by breast cancer cells will lead to their direct in vivo destruction by anti-alpha Ga1 antibodies and complement, and boost the immune response to other tumor antigens. In vitro transduction of MCF-7, and T47D human breast cancer cells with an HSV amplicon vector resulted in expression of the suicide gene as detected by specific binding of labeled IB-4 isolectin.
- Genetic Engineering and Molecular Biology
- Medicine and Medical Research