Blocking Internalization of Phosphatidylethanolamine at Cleavage Furrow of Mitosis as a Novel Mechanism of Anti-Breast Cancer Strategy
Annual rept. 1 Jun 2001-31 May 2002
WAKE FOREST UNIV WINSTON-SALEM NC SCHOOL OF MEDICINE
Pagination or Media Count:
During the formation of cleavage furrow of mitosis, phosphatidylethanolamine PE flips from inner leaflet of the plasma membrane to the outer leaflet specifically in the furrow region near the contractile ring. immediately after the contractile ring separates the two daughter cells, PE returns from outer leaflet to inner leaflet. This transient movement of PE during cytokinesis is essential because blockage of this PE movement results in a failure of mitosis and leads to cell death. Cinnamycin produced by Streptoverticillium griseoverticillatum targets specifically to PE on cell surface at the cleavage furrow of mitotic cells but not the non-dividing cells. This proposal is to test if cinmamycin is a better anti-tumor drug for treatment of breast cancers because of several advantages 1 Cinmamycin only targets proliferating cells but has no effect on non- proliferating cells. 2 The anti-proliferation activity doesnt require cinnamycin to enter the cells. 3 Cinnamycin doesnt have to suffer the effect of multi-drug resistance mechanism or cellular metabolism. because cinnamycin is no longer available commercially, we had to devise production procedures and to purify this compound in our own lab. Thus, completion of this proposal would require longer time than that was originally proposed.
- Medicine and Medical Research