Isolation and Characterization of NF1 Target Genes
Annual rept. 1 Sep 2001-31 Aug 2002
COLD SPRING HARBOR LAB NY
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One of the most defining clinical features of the Neurofibromatosis type 1 NFl disease is the widespread occurrence of multiple benign, peripheral nerve tumors, called neurofibromas, which in some cases can further progress to malignancies. A well-defined explanation of the development of neurofibromas at the molecular level is still lacking. Whereas deregulation of the Ras-mediated signaling pathway contributes to the pathology of NFl, evidence for the involvement of Ras-independent pathways has also been provided. The nature of these pathways remains however to be defined. To gain more insights into the identity of these signaling pathways, we proposed to utilize cDNA microarrays to profile the expression patterns of NF1-associated tumor cells. We set out to isolate neurofibroma and wild type Schwann cells derived from different patients. cDNA representations prepared from these cells will be used as pairs in our profiling experiments using a microarray chip containing 25,000 human cDNA probes. In addition, it is our intent to perform profiling experiments using neurofibroma and wild type Schwann cells inducibly expressing NF1 to assure that the differences observed are indeed due to NF1. The generation of neurofibroma and wild type Schwann cells inducibly expressing NF1 is currently underway.
- Anatomy and Physiology
- Medicine and Medical Research