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Structure/Function Studies of the Androgen Receptor DNA-Binding Region
Annual rept. 1 Apr 2001-31 Mar 2002
VIRGINIA UNIV CHARLOTTESVILLE
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In this annual report we discuss the progress over the past 12 months towards characterizing the structure-function relationship in the androgen receptor AR DNA-binding domain DBD. We have over-produced large quantities of the AR-DBD using E. coli expression systems, and obtained pure preparations of DNA targets of various size and composition, all of which incorporate the AR binding site. We continue to work towards obtaining the highest resolution X-ray diffraction data from crystals of the AR-DBDDNA complex and have been optimizing our crystalline preparations. In addition, we have made progress toward characterizing previously unknown functions of the AR-DBD. We report in two manuscripts completed in the previous 12 months included in the appendix our findings that the DBD acts as the nuclear export signal for AR and related nuclear receptors, and that this export is dependent on Calreticuling and calcium.
APPROVED FOR PUBLIC RELEASE