Annual summary rept. 1 Aug 2001-31 Jul 2002
DUKE UNIV MEDICAL CENTER DURHAM NC
Pagination or Media Count:
Initial characterization of the ability of the human Reaper homology hRpr to initiate apoptosis in human cell lines has proven to be difficult. Possible explanations for the difficulty in working with hRpr include low expression levels and the potential requirement for a second protein to cooperate or synergize with hRpr in order to initiate apoptosis. However, many of the goals set out in the statement of work have been achieved, including the construction of hRpr expression vectors and successful transfection of hRpr into human cell lines. Additionally, numerous apoptosis assays have been perfected, setting the stage for successful analysis once the correct conditions are for hRpr expression and activity are worked out. Furthermore, research that deviated from the original statement of work has led to a publication and strengthened my ability to characterize hRpr. For example, studies of Reaper itself have revealed that the interaction between Reaper and lAPs results in the ubiquitination and degradation of both proteins. This proteolytic regulation of Reaper may also explain the difficulty expressing hRpr. Additionally, we have found that Reaper inhibits translation, which opens up another exciting avenue for characterizing the activity of hRpr.
- Medicine and Medical Research