Accession Number:

ADA409648

Title:

Mammary-Specific Gene Transfer for Modeling Breast Cancer

Descriptive Note:

Annual summary 1 Aug 2001-31 Jul 2002

Corporate Author:

MEMORIAL SLOAN-KETTERING CANCER CENTER NEW YORK

Personal Author(s):

Report Date:

2002-08-01

Pagination or Media Count:

8.0

Abstract:

The TVA retroviral gene transfer system allows for the examination of multiple genetic lesions in vivo without the need to create and breed individual transgenic lines. The system is based on the use of the RCAS virus an avian leukosis virus vector of subgroup A to deliver genes to mammalian cells or tissues that have been engineered to produce the avian viral receptor TVA. Transgenic mice have been generated to express TVA in the mammary gland. Mammary tumors can be induced in these mice by in vivo infection of mammary glands with virus encoding polyoma middle T antigen. Mammary cells, isolated from the TVA mice that have been bred to p53 nullizygosity, can be infected ex vivo with RCAS vectors expressing oncogenes, and the transplantation of the infected cells into the fat pad of non-transgenic mice results in rapid development of mammary tumors. This somatic gene delivery system may be useful for dissecting genetic interactions that operate in breast cancer.

Subject Categories:

  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE