Protein Microarray Technology for the Noninvasive Diagnosis and Prognosis of Breast Cancer
Annual rept. 1 Jul 2001-30 Jun 2002
BATTELLE PACIFIC NORTHWEST LABS RICHLAND WA
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A number of circulating markers have been identified that have the potential to be used in the detection or prognosis of breast cancer. Unfortunately, no single marker is consistently increased in breast cancer patients when compared with the general population. We hypothesized, however, that a sophisticated analysis of large number of circulating markers would accurately detect breast cancer as well as provide a valuable tool for prognosis. Therefore, we proposed to develop a rapid and simple system to measure a large number of blood markers associated with breast cancer. We will accomplish this by developing an antibody microarray with antibodies specific to different blood markers. Currently, we have screened fourteen markers, and generated standard curves for eight of these markers. We have refined the microarray to measure markers with a sensitivity down to 0.5 pgml. This sensitivity will allow the use of this microarray to screen up to 200 serum samples from breast cancer patients and control patients. These data will be analyzed using sophisticated computer programs that are designed to find relationships in a complex data set such as this. These studies will result in a prototype chip that can be used for the rapid determination of circulating markers associated with breast cancer.
- Medicine and Medical Research