Induction of Human Somatostatin Receptor Subtype 2 on Breast Tumors with an Adenoviral Vector for Their Treatment and Detection with a Radiolabeled Peptide
Final rept. 1 Jun 2001-31 May 2002
ALABAMA UNIV IN BIRMINGHAM
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Targeted radiation therapy for breast cancer is a rational approach, however there are problems associated with low receptor expression on tumors and bone marrow toxicity due to the long serum half-life of antibodies used to target the radiation. An adenoviral vector encoding the human somatostatin receptor subtype 2 AdSSTr2 has been produced. The MDA- MB-468 and BT-474 human breast cancer cells were infected with AdSSTr2 and harvested 48 h later for membrane preparations. Membrane SSTr2 expression was determined by a competitive binding assay using iodine-l25 1-125-labeled somatostatin. Localization of Tc-99m-P2O45 a high affinity somatostatin analogue in mice bearing subcutaneous tumors was evaluated by injecting AdSSTr2 i.v. followed by an i.v. injection of Tc-99m-P2O45 48 h later. Localization of Tc-99m-P2O45 was determined by counting tissues in a gamma counter. These studies provide the proof-of-principle that this novel paradigm can be used to treat and detect breast cancer.
- Medicine and Medical Research