Identification of Hepatocyte Growth Factor Autocrine Loops in Breast Carcinomas: Possible Target for Therapeutic Intervention
Annual summary rept. 1 Jul 2001-1 Jul 2002
QUEEN'S UNIV KINGSTON (ONTARIO)
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Hepatocyte growth factor HGF binding to its transmembrane receptor, Met, results in an increase in breast epithelial cell motility, morphogenesis and metastasis. HGF-like forms have been detected as secretion products of the human breast epithelial carcinoma cell line, MCF10A1T3B. The two major products are 56 and 32 kDa. Recombinant HGF treatment with elastase produced the 56 kDa HGF antagonistic product, an NK4-like form. Anion exchange chromatography of conditioned medium indicated that MCF10A1T3B secretion products have decreased affinity compared to endogenous full-length HGF.