Involvement of a Human Endogenous Retrovirus in Breast Cancer
Annual rept. 1 Aug 2001-31 Jul 2002
TULANE UNIV HEALTH SCIENCE CENTER NEW ORLEANS LA
Pagination or Media Count:
The genomic DNA of a subset of humans contains an endogenous retrovirus closely related to mouse mammary tumor virus MWTV . Our overall goal is to determine whether the human mammary tumor virus HMTV sequences are involved in a subset of human breast cancers. The first specific aim of this proposal is to recruit and clinically characterize cohorts of breast cancer and appropriate control patients. In studies proposed in the second specific aim, we will identify and sequence HMTV nucleic acids in breast cancer tissue, control tissue, and blood of patients from our cohorts. We will also determine the incidence of HMTV in these various control populations, and compare the sequences of several HMTV genes from different individuals to determine the extent of genetic variability. The third specific aim is to construct DNA or cDNA libraries from tissues positive for HMTV proviruses. In studies under specific aim four, we propose to express HMTV proteins in an insect cell system, which allows stable expression of recombinant proteins, and to characterize the immunological reactions of breast cancer patients and controls against HMTV proteins. If a definitive link is established, HMTV will provide a target for vaccine development and breast cancer therapy.
- Medicine and Medical Research