Accession Number:

ADA409078

Title:

Towards the Batch Synthesis of Long DNA

Descriptive Note:

Final rept. Jan-Aug 2002

Corporate Author:

INSTITUTE FOR DEFENSE ANALYSES ALEXANDRIA VA

Personal Author(s):

Report Date:

2002-10-01

Pagination or Media Count:

87.0

Abstract:

Production-scale synthesis of long DNAs with specific base sequences by conventional methods is generally infeasible due to error rates On the other hand, sticky-ended double-stranded ds DNA complexes can be joined into a single dsDNA complex with an enzyme called DNA ligase This idea offers a way to indirectly synthesize very long 10kb DNAs-by hybridization of a batch of single-stranded ss DNAs followed by ligation-with predetermined base sequences We analyze such synthesis protocols In the natural course of this development, we take a reasonably self-contained tour through much of what is known about the physics of DNA hybridization as it relates to the issues here This paper is therefore a primer on DNA self-assembly, using the staggered ligation model for DNA synthesis as the working example

Subject Categories:

  • Biochemistry
  • Genetic Engineering and Molecular Biology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE