Accession Number:
ADA409078
Title:
Towards the Batch Synthesis of Long DNA
Descriptive Note:
Final rept. Jan-Aug 2002
Corporate Author:
INSTITUTE FOR DEFENSE ANALYSES ALEXANDRIA VA
Personal Author(s):
Report Date:
2002-10-01
Pagination or Media Count:
87.0
Abstract:
Production-scale synthesis of long DNAs with specific base sequences by conventional methods is generally infeasible due to error rates On the other hand, sticky-ended double-stranded ds DNA complexes can be joined into a single dsDNA complex with an enzyme called DNA ligase This idea offers a way to indirectly synthesize very long 10kb DNAs-by hybridization of a batch of single-stranded ss DNAs followed by ligation-with predetermined base sequences We analyze such synthesis protocols In the natural course of this development, we take a reasonably self-contained tour through much of what is known about the physics of DNA hybridization as it relates to the issues here This paper is therefore a primer on DNA self-assembly, using the staggered ligation model for DNA synthesis as the working example
Descriptors:
Subject Categories:
- Biochemistry
- Genetic Engineering and Molecular Biology