Patterning Micro- and Nanometer Scale Lipid Bilayers Using a Polymer-Based Lift-Off
CORNELL UNIV ITHACA NY SCHOOL OF APPLIED AND ENGINEERING PHYSICS
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Lipids, antibodies, antigen, avidin and biotin have been patterned at nanoscale resolution for the precise immobilization and stimulation of immunological cells. We demonstrate that biomaterial can be patterned on silicon using a photolithographically patterned polymer lift-off technique. The nanoscale pattern is realized as the polymer is mechanically peeled away in one contiguous piece in solution. The 600 nm to 67 microns biomaterial patches provide a synthetic biological substrate for biochemical analysis. 100-nm unilamellar lipid vesicles spread to form a supported lipid bilayer on thermally oxidized silicon surface as confirmed by fluorescence recovery after photobleaching FRAP This method provides a new technique for immobilizing biomaterials, capturing antibodies from solution, and providing a platform for nanoscale antigenic stimulus.
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