Laser Bioeffects: Differential Protein Expression of Cultured Human Melanocytes Treated With 532 nm Picosecond Pulse Laser-Light
AIR FORCE INST OF TECH WRIGHT-PATTERSONAFB OH
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The use of laser light for targeting devices and industry has dramatically increased the likelihood that personnel will be exposed to laser energy during military and commercial operations. The increased potential for exposure of humans to lasers highlights the need to understand laser-tissue interactions at the most basic cell and molecular levels. Current ultrashort pulse laser safety standards are based on a minimal visible lesion MVL, i.e., histological, damage endpoint in the Rhesus monkey model Shaver, 2001. A human model for assessing laser-light damage to tissue at the cell and molecular level is desirable for scientific, political and fiduciary reasons. This research assesses the effects of sublethal pulsed laser-light treatment to a human melanocyte skin cell line. Proteomic inquiry into the cellular effect of laser treatment was accomplished via parallel sample analysis with over 850 monoclonal antibodies in order to screen for changes in the levels of protein expression. In short, the data indicated dramatically increased molecular debridement, decreased apoptotic signaling and cell cycling and other cellular phenomena sufficient to propose a stress response and recovery paradigm.
- Lasers and Masers