Accession Number:

ADA399591

Title:

Evaluation of an In Vitro of Human Immune Activation Induced by Freeze-Thaw Tissue Damage

Descriptive Note:

Technical rept.

Corporate Author:

ARMY RESEARCH INST OF ENVIRONMENTAL MEDICINE NATICK MA

Personal Author(s):

• DuBose, D. A.
• Rufolo, D. M.
• Morehouse, D. H.

Report Date:

2002-02-01

Pagination or Media Count:

24.0

Abstract:

In training and in combat, soldiers are under the constant threat of injury. Injury that results in tissue necrosis can activate the immune system and ultimately enhance disturbances in organ function. Knowledge of the immune activation mechanisms may lead to methods of reducing tissue damage. Mechanistic studies require precise control over the complex factors that coordinate the tissue injury cascade. An in vitro model would provide such a level of control. However, the feasibility of obtaining the required immune system tissues, such as human whole blood HWB, endothelial cells and keratinocytes from the same human donor is unlikely. As such, an in vitro model comprised of tissues from immunologically distinct donors is perhaps the next best approach, but this necessitates an experimental design that controls for adverse histocompatibility or tissue rejection reactions. The current study employed permeable membranes to physically separated immunologically distinct immune system tissues.

Descriptors:

• *TISSUES(BIOLOGY)
• *IMMUNITY
• RESPONSE(BIOLOGY)
• CELLS(BIOLOGY)
• ANTIGENS
• NECROSIS
• EPIDERMIS
• ENDOTHELIUM
• HISTOCOMPATIBILITY
• LYMPH NODES
• FROSTBITE
• LYMPHATIC SYSTEM

Subject Categories:

• Anatomy and Physiology
• Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE