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Luminescent Nanoparticles for High Sensitivity Biosensing
Final rept. 28 Mar 2000-30 Sep 2001
NAVAL RESEARCH LAB WASHINGTON DC
Pagination or Media Count:
Water-soluble luminescent nanoparticles have been fabricated with suitable surface chemistry to attach biomolecules, engineered to attach via spontaneous self-assembly. The resulting bio-conjugates have been characterized and the effects of pH and ionic strength on stability examined. Additionally, methods to improve bacterial expression and purification of the fusion proteins have been developed. QDs coated with protein G, which binds antibody, have been utilized in developing sensitive immunoassays. Finally, viral capsids, which are made of repeating subunits, are being utilized as signal amplifiers, enhancing the sensitivity of optical sensors with the goal of detecting single molecular events.
APPROVED FOR PUBLIC RELEASE