Accession Number:

ADA399090

Title:

Tumor Targeting With Phage Library

Descriptive Note:

Annual summary rept. 1 Jul 1999-30 Jun 2001

Corporate Author:

BURNHAM INST LA JOLLA CA

Personal Author(s):

Report Date:

2001-07-01

Pagination or Media Count:

6.0

Abstract:

I have proposed to identify peptides that bind to the vasculature of prostate cancers by using a technique developed in our laboratory called in vivo phage display. These peptides would have the potential to be used in drug targeting. The tumor-specific peptides will then be used to isolate their vasculature-specific receptors. The receptors could be specific markers for the vasculature of prostate cancers and lead to a greater understanding of what kinds of vascular surface proteins are up-regulated during prostate carcinogenesis. Because of the difficulty in the experimental procedure, I took an alternative approach, mRNA profiling by GeneChip or cDNA microarray. To this end, I have accomplished the following goals 1 Established GeneChip and cDNA Microarray systems in our laboratory. 2 Established an in vitro transcription method to amplify limiting amount of RNA that can be obtained from mice. 3 Improved endothelial cell purification procedures by adopting magnetic beads to increase yield. 4 Screened endothelial cell specific markers from lung and kidney.

Subject Categories:

  • Biochemistry
  • Genetic Engineering and Molecular Biology
  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE