Accession Number:

ADA399013

Title:

Biomarkers for Detection of In Vitro Ocular Irritancy

Descriptive Note:

Final rept. 1 Mar 1998-28 Feb 2001

Corporate Author:

LYNNTECH INC COLLEGE STATION TX

Personal Author(s):

• Eurell, Thomas E.

Report Date:

2001-05-01

Pagination or Media Count:

13.0

Abstract:

Exposure of rabbit corneas to nanosecond pulse width 1540 nm infrared laser light produced a less severe coagulative necrosis when compared to millisecond pulse width exposures. We expected this result because nanosecond pulse widths should have less of a photothermal effect than millisecond pulse widths. Matrix Metalloproteinase-2 MMP-2 immunohistochemistry was used to detect if subtle stromal remodeling had occurred. We found a markedly increased MMP-2 immunohistochemical pattern than what we would have predicted from the HE stains alone. Approximately half of the beam path was filled with MMP-2 reaction product. As far as we know this is the first analysis comparing the stromal remodeling effects of an infrared laser operating in both the millisecond and nanosecond pulse widths. This finding is important to the AFOSR mission because it demonstrates that significant tissue changes associated with comeal wound healing can occur following exposure to nanosecond laser pulse widths that are not detectable with standard histologic techniques.

Descriptors:

• *PORTABLE EQUIPMENT
• *PULSED LASERS
• *IN VITRO ANALYSIS
• *INFRARED LASERS
• *NECROSIS
• DETECTION
• EXPOSURE(GENERAL)
• PATHS
• PULSE RATE
• WOUNDS AND INJURIES
• MARKERS
• INFRARED RADIATION
• HEALING
• HISTOLOGY
• RABBITS
• IMMUNOCHEMISTRY
• TRACER STUDIES
• HISTOCHEMISTRY
• CORNEA
• PHOTOTHERMAL PROPERTIES

Subject Categories:

• Anatomy and Physiology
• Lasers and Masers
• Logistics, Military Facilities and Supplies

Distribution Statement:

APPROVED FOR PUBLIC RELEASE