Use of Intraductal Adenovirus Transduction to Assess the Mammary Tumorigenic Potential of a Constitutively Active Prolactin Receptor
COLORADO UNIV HEALTH SCIENCES CENTER DENVER
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We have established adenovirus transduction as a method for the short term alteration of gene expression in the mammary epithelium. Detailed conditions for transducing up to 30 of the cells in the mammary gland of the late pregnant mouse have been worked out, transduction with adenovirus and expression of green fluorescent protein, OFP, have been shown not to interfere with function of the mammary cells in which they are expressed. The transgene is maintained for seven days in the lactating mouse after transduction in late pregnancy without inflammation and without interfering with the function of the gland. Within this time frame function altering transgenes can be studied. however, longer term transduction is associated with significant inflammation. Transgenic technology has been used to evaluate the effects of an activated prolactin receptor, aPRL, and an activated member of the prolactin signal transduction pathway, Akt on the mammary epithelium. The latter has been shown to prolong mammary involution and preliminary results suggest it may enhance tumorigenesis. Adenovirus transduction is appropriate for short term experiments, up to 7 days, but cannot be used for long term expression of foreign proteins because of inflammatory reactions.
- Medicine and Medical Research