Control of Expression of Insulin-Like Growth Factor II in Stromal Cells of Breast Cancer
Annual summary rept. 1 Jul 2000-30 Jun 2001
GEORGETOWN UNIV WASHINGTON DC MEDICAL CENTER
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Our hypothesis is that the IGF2 gene is methylated in normal breast stromal cells, and that a decrease in methylation results in increase in stromal IGF2 expression in the breast malignancy, enhancing tumor growth. This work analyzes the biochemical mechanisms that alter methylation of the IGF2 promoter in benign stromal cells adjacent to the malignant epithelial cells in the breast tumor. The studies of this project were conducted in two systems T6 1 breast cancer system, an ER, PR, IGF2 expressing breast cancer xenograft passaged in nude mice, and matched fibroblast sets from breast cancer patients. These studies could provide critical insight into stromal regulation of breast cancer and provide a new target for therapy.
- Genetic Engineering and Molecular Biology
- Medicine and Medical Research