The Prostate Expression Database
Annual rept. 1 Apr 2000-31 Mar 2001
FRED HUTCHINSON CANCER RESEARCH CENTERSEATTLE WA
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This proposal aims to exploit advances in biotechnology and informatics to develop a genetics resource termed the Prostate Expression Database PEDB httpwww.pedb.org. The foundation of PEDB is the identification and characterization of a prostate transcriptome, the intermediary between the genome and the proteome that represents that portion of the human genome actively used or transcribed in the prostate. The research accomplished to date has assembled a working virtual prostate transcriptome that defines the genes used or transcribed in prostate cell types and tissues. This transcriptome has a physical counterpart of 6,000 cDNAs arrayed in cDNA microarray format for large-scale expression studies. This transcriptome has been used as a foundation for studies of the prostate proteome, the working counterpart to the genome and transcriptome. Our results show that these approaches are complementary. Analysis of the virtual transcriptome of LNCaP cells has identified 13 new androgen-regulated genes to date. Characterization of these genes is in progress. One gene, PSDR1, exhibits sequence homology with a family of proteins involved in steroid hormone metabolism, and may modulate steroid activity within the prostate.
- Medicine and Medical Research
- Computer Programming and Software